Fig. 5. NR inhibits tumor growth in HCT116 xenografts. (A) Representative picture of tumors excised from mice injected with PBS, 10 mg/kg NR (NR10), 50 mg/kg NR (NR50) and 25 mg/kg 5-FU (5-FU). (B) The volume of tumors was measured during indicated periods. Data are tumor volume mean ± SD, and each groups were eight mice (n =8). Two of eight animals in 5-FU group were unexpectedly died after 30 days. Survival rate is 100% (8/8) in control, 100% (8/8) NR50, and 75% (6/8) in 5-FU. (C) Average tumor weight excied from each group. (D) Average body weight were recorded for 40 days after drug injection. Error bars represent SD, *p<0.05. (E) Immunohistochemistry of β-catenin (top) and USP4 (bottom) levels in the xenograft tumor tissues of mice were observed under ×20 and ×40 magnification and added scale bar in the bottom. (F) Expression of β-catenin protein in the xenograft tumors. Two representative tumor tissues from each group (control, NR50, and 5-FU) were randomly homogenized in lysis buffer and subjected to Western blotting with specific anti-β-catenin antibody. β-actin was used as a loading control.